Transcriptome analysis of the copepod Eurytemora affinis upon exposure to endocrine disruptor pesticides: Focus on reproduction and development.
Identifieur interne : 000033 ( Main/Exploration ); précédent : 000032; suivant : 000034Transcriptome analysis of the copepod Eurytemora affinis upon exposure to endocrine disruptor pesticides: Focus on reproduction and development.
Auteurs : Eléna Legrand [France] ; Joëlle Forget-Leray [France] ; Aurélie Duflot [France] ; Stéphanie Olivier [France] ; Jean-Pierre Thomé [Belgique] ; Jean-Michel Danger [France] ; Céline Boulangé-Lecomte [France]Source :
- Aquatic toxicology (Amsterdam, Netherlands) [ 1879-1514 ] ; 2016.
Abstract
Copepods-which include freshwater and marine species-represent the most abundant group of aquatic invertebrates. Among them, the calanoid copepod Eurytemora affinis is widely represented in the northern hemisphere estuaries and has become a species of interest in ecotoxicology. Like other non-target organisms, E. affinis may be exposed to a wide range of chemicals such as endocrine disruptors (EDs). This study investigated the gene expression variation in E. affinis after exposure to ED pesticides-chosen as model EDs-in order to (i) improve the knowledge on their effects in crustaceans, and (ii) highlight relevant transcripts for further development of potential biomarkers of ED exposure/effect. The study focused on the reproduction function in response to ED. Copepods were exposed to sublethal concentrations of pyriproxyfen (PXF) and chlordecone (CLD) separately. After 48h, males and females (400 individuals each) were sorted for RNA extraction. Their transcriptome was pyrosequenced using the Illumina(®) technology. Contigs were blasted and functionally annotated using Blast2GO(®). The differential expression analysis between ED- and acetone-exposed organisms was performed according to sexes and contaminants. Half of the 19,721 contigs provided by pyrosequencing were annotated, mostly (80%) from arthropod sequences. Overall, 2,566 different genes were differentially expressed after ED exposures in comparison with controls. As many genes were differentially expressed after PXF exposure as after CLD exposure. In contrast, more genes were differentially expressed in males than in females after both exposures. Ninety-seven genes overlapped in all conditions. Finally, 31 transcripts involved in reproduction, growth and development, and changed in both chemical exposures were selected as potential candidates for future development of biomarkers.
DOI: 10.1016/j.aquatox.2016.04.010
PubMed: 27111276
Affiliations:
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Electronic address: elena.legrand@univ-lehavre.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Normandy University, ULH, UMR-I 02 INERIS, Environmental Stresses and Biomonitoring of Aquatic Ecosystems (SEBIO)-SFR SCALE 4116, F-76600 Le Havre</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Forget Leray, Joelle" sort="Forget Leray, Joelle" uniqKey="Forget Leray J" first="Joëlle" last="Forget-Leray">Joëlle Forget-Leray</name><affiliation wicri:level="3"><nlm:affiliation>Normandy University, ULH, UMR-I 02 INERIS, Environmental Stresses and Biomonitoring of Aquatic Ecosystems (SEBIO)-SFR SCALE 4116, F-76600 Le Havre, France. Electronic address: joelle.leray@univ-lehavre.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Normandy University, ULH, UMR-I 02 INERIS, Environmental Stresses and Biomonitoring of Aquatic Ecosystems (SEBIO)-SFR SCALE 4116, F-76600 Le Havre</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Duflot, Aurelie" sort="Duflot, Aurelie" uniqKey="Duflot A" first="Aurélie" last="Duflot">Aurélie Duflot</name><affiliation wicri:level="3"><nlm:affiliation>Normandy University, ULH, UMR-I 02 INERIS, Environmental Stresses and Biomonitoring of Aquatic Ecosystems (SEBIO)-SFR SCALE 4116, F-76600 Le Havre, France. Electronic address: aurelie.duflot@univ-lehavre.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Normandy University, ULH, UMR-I 02 INERIS, Environmental Stresses and Biomonitoring of Aquatic Ecosystems (SEBIO)-SFR SCALE 4116, F-76600 Le Havre</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Olivier, Stephanie" sort="Olivier, Stephanie" uniqKey="Olivier S" first="Stéphanie" last="Olivier">Stéphanie Olivier</name><affiliation wicri:level="3"><nlm:affiliation>Normandy University, ULH, UMR-I 02 INERIS, Environmental Stresses and Biomonitoring of Aquatic Ecosystems (SEBIO)-SFR SCALE 4116, F-76600 Le Havre, France. Electronic address: stephanie.olivier@univ-lehavre.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Normandy University, ULH, UMR-I 02 INERIS, Environmental Stresses and Biomonitoring of Aquatic Ecosystems (SEBIO)-SFR SCALE 4116, F-76600 Le Havre</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Thome, Jean Pierre" sort="Thome, Jean Pierre" uniqKey="Thome J" first="Jean-Pierre" last="Thomé">Jean-Pierre Thomé</name><affiliation wicri:level="3"><nlm:affiliation>University of Liège, Laboratory of Animal Ecology and Ecotoxicology (LEAE), Centre for Analytical Research and Technology (CART), 4000 SART-Tilman, Belgium. Electronic address: jp.thome@ulg.ac.be.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>University of Liège, Laboratory of Animal Ecology and Ecotoxicology (LEAE), Centre for Analytical Research and Technology (CART), 4000 SART-Tilman</wicri:regionArea><placeName><region type="province" nuts="2">Province de Liège</region><settlement type="city">Liège</settlement></placeName></affiliation></author><author><name sortKey="Danger, Jean Michel" sort="Danger, Jean Michel" uniqKey="Danger J" first="Jean-Michel" last="Danger">Jean-Michel Danger</name><affiliation wicri:level="3"><nlm:affiliation>Normandy University, ULH, UMR-I 02 INERIS, Environmental Stresses and Biomonitoring of Aquatic Ecosystems (SEBIO)-SFR SCALE 4116, F-76600 Le Havre, France. Electronic address: jean-michel.danger@univ-lehavre.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Normandy University, ULH, UMR-I 02 INERIS, Environmental Stresses and Biomonitoring of Aquatic Ecosystems (SEBIO)-SFR SCALE 4116, F-76600 Le Havre</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Boulange Lecomte, Celine" sort="Boulange Lecomte, Celine" uniqKey="Boulange Lecomte C" first="Céline" last="Boulangé-Lecomte">Céline Boulangé-Lecomte</name><affiliation wicri:level="3"><nlm:affiliation>Normandy University, ULH, UMR-I 02 INERIS, Environmental Stresses and Biomonitoring of Aquatic Ecosystems (SEBIO)-SFR SCALE 4116, F-76600 Le Havre, France. Electronic address: celine.lecomte@univ-lehavre.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Normandy University, ULH, UMR-I 02 INERIS, Environmental Stresses and Biomonitoring of Aquatic Ecosystems (SEBIO)-SFR SCALE 4116, F-76600 Le Havre</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author></analytic><series><title level="j">Aquatic toxicology (Amsterdam, Netherlands)</title><idno type="eISSN">1879-1514</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Copepods-which include freshwater and marine species-represent the most abundant group of aquatic invertebrates. Among them, the calanoid copepod Eurytemora affinis is widely represented in the northern hemisphere estuaries and has become a species of interest in ecotoxicology. Like other non-target organisms, E. affinis may be exposed to a wide range of chemicals such as endocrine disruptors (EDs). This study investigated the gene expression variation in E. affinis after exposure to ED pesticides-chosen as model EDs-in order to (i) improve the knowledge on their effects in crustaceans, and (ii) highlight relevant transcripts for further development of potential biomarkers of ED exposure/effect. The study focused on the reproduction function in response to ED. Copepods were exposed to sublethal concentrations of pyriproxyfen (PXF) and chlordecone (CLD) separately. After 48h, males and females (400 individuals each) were sorted for RNA extraction. Their transcriptome was pyrosequenced using the Illumina(®) technology. Contigs were blasted and functionally annotated using Blast2GO(®). The differential expression analysis between ED- and acetone-exposed organisms was performed according to sexes and contaminants. Half of the 19,721 contigs provided by pyrosequencing were annotated, mostly (80%) from arthropod sequences. Overall, 2,566 different genes were differentially expressed after ED exposures in comparison with controls. As many genes were differentially expressed after PXF exposure as after CLD exposure. In contrast, more genes were differentially expressed in males than in females after both exposures. Ninety-seven genes overlapped in all conditions. Finally, 31 transcripts involved in reproduction, growth and development, and changed in both chemical exposures were selected as potential candidates for future development of biomarkers.</div></front></TEI><affiliations><list><country><li>Belgique</li><li>France</li></country><region><li>Haute-Normandie</li><li>Province de Liège</li><li>Région Normandie</li></region><settlement><li>Le Havre</li><li>Liège</li></settlement></list><tree><country name="France"><region name="Région Normandie"><name sortKey="Legrand, Elena" sort="Legrand, Elena" uniqKey="Legrand E" first="Eléna" last="Legrand">Eléna Legrand</name></region><name sortKey="Boulange Lecomte, Celine" sort="Boulange Lecomte, Celine" uniqKey="Boulange Lecomte C" first="Céline" last="Boulangé-Lecomte">Céline Boulangé-Lecomte</name><name sortKey="Danger, Jean Michel" sort="Danger, Jean Michel" uniqKey="Danger J" first="Jean-Michel" last="Danger">Jean-Michel Danger</name><name sortKey="Duflot, Aurelie" sort="Duflot, Aurelie" uniqKey="Duflot A" first="Aurélie" last="Duflot">Aurélie Duflot</name><name sortKey="Forget Leray, Joelle" sort="Forget Leray, Joelle" uniqKey="Forget Leray J" first="Joëlle" last="Forget-Leray">Joëlle Forget-Leray</name><name sortKey="Olivier, Stephanie" sort="Olivier, Stephanie" uniqKey="Olivier S" first="Stéphanie" last="Olivier">Stéphanie Olivier</name></country><country name="Belgique"><region name="Province de Liège"><name sortKey="Thome, Jean Pierre" sort="Thome, Jean Pierre" uniqKey="Thome J" first="Jean-Pierre" last="Thomé">Jean-Pierre Thomé</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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